Binding interactions of actinomycin D anticancer drug with bile salts micelles

Interactions of unconjugated bilirubin with bile salts.

The rate of peroxidation of unbound, unconjugated bilirubin (UCB) was used to assess the interactions of UCB with four taurine-conjugated bile salts at pH 8.2, 37 degrees C, and an ionic strength of 0.15. Each of the four structurally different bile salts markedly decreased the rate of peroxidation of UCB in the presence of horseradish peroxidase (HRP); 30% of UCB was bound even at low, premice...

Surface Effects on DNA-Drug Interactions: Characterization of Actinomycin D Binding by Surface Plasmon Resonance

Actinomycin D-deoxynucleotide interactions: binding isotherms at the benzenoid and quinoid portions of the drug.

Titrations of actinomycin D (AMD) with dG and with dG-dC were monitored by circular dichroism at 380 nm and 470 nm. These wavelengths are sensitive predominantly to nucleotide binding processes at the benzenoid and quinoid portions, respectively, of the phenoxazone ring of the drug chromophore [Auer, H.E., Pawlowski-Konopnicki, B.E., Chiao, Y.C.C. & Krugh, T.R. (1978), Biopolymers, 17, 1891-191...

Interactions of pancreatic lipase with bile salts and dodecyl sulfate.

Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is paralle...

Intestinal Probiotics: Interactions with Bile Salts and Reduction of Cholesterol

Probiotics mainly reside in host intestinal tract, where they interact with bile salts and ingested cholesterol. This paper reviews the recent progresses of probiotics on the following issues, i.e. bile salts effect on membrane composition; probiotic tolerance and modification of bile salts; the mechanism of probiotic removal of host cholesterol; and the roles that bile salt hydrolase plays. © ...